Table of Contents
What is aseptic transfer?
Finally, in working with microorganisms, we must have a method of transferring growing organisms (called the inoculum) from a pure culture to a sterile medium without introducing any unwanted outside contaminants. This method of preventing unwanted microorganisms from gaining access is termed aseptic technique.
What are the aseptic techniques?
Aseptic technique is a collection of medical practices and procedures that helps protect patients from dangerous germs. Bacteria, viruses, and microorganisms are everywhere, so using aseptic technique can help keep important equipment from being contaminated.
What Is culture transfer technique?
PURPOSE. Technique of aseptic removal and transfer of microorganisms for sub-culturing. PRINCIPLE. Microorganisms are transferred from one medium to another by subculturing.
Which of the following are the smallest microorganisms that need a host to supply food and an environment in which to multiply?
Viruses are the smallest and simplest microorganisms. Unlike bacteria, yeasts, and molds, viruses are incapable of reproducing independently. Instead, they must first invade the cells of another living organism called the host, before they can multiply. Hence, they are parasitic.
What is a sterile technique?
Sterile technique. Sterile technique involves strategies used in patient care to reduce exposure to microorganisms and maintain objects and areas as free from microorganisms as possible.
How are microorganisms spread on a plate?
The solution is then spread uniformly through a number of possible means, the most popular is the use of sterile glass beads that are shook on top of the media, spreading the microbe-containing liquid evenly on the plate. Also common is the use of a bent-glass rod, often referred to as a hockey stick, due to its similar shape.
How can micro-organisms be detected by looking under a microscope?
When examining foods, the possibility of detecting the presence of micro-organisms by looking at a sample directly under the microscope should not be missed. A small amount of material can be mounted and teased out in a drop of water on a slide, covered with a cover slip, and examined, first with a low magnification, and then with a x 45 objective.
What are the methods for the detection of toxins and organisms?
The methods are:- 1. Indicator Organisms 2. Direct Examination 3. Cultural Techniques 4. Enumeration Methods 5. Alternative Methods 6. Rapid Methods for the Detection of Specific Organisms and Toxins 7. Laboratory Accreditation. Method # 1. Indicator Organisms:
What are the methods for the microbiological examination of foods?
The following points highlight top seven methods for the microbiological examination of foods. The methods are:- 1. Indicator Organisms 2. Direct Examination 3. Cultural Techniques 4. Enumeration Methods 5. Alternative Methods 6. Rapid Methods for the Detection of Specific Organisms and Toxins 7. Laboratory Accreditation.