Which pigment moves the slowest in spinach leaf?

Which pigment moves the slowest in spinach leaf?

Yellow-green chlorophyll b travels the least distance with the mobile phase. Chlorophyll b is a more polar (water-loving) pigment than the other pigments found in spinach extracts and is therefore more strongly attracted to the polar surface of the paper than to the nonpolar solvent.

Which plant pigments would you expect to travel the slowest in paper chromatography?

Which pigment traveled the slowest? why did it travel slower than the other pigments? Chlorophyll B , was the slowest because it was the most polar and was attacted to the polar paper.

Which pigment in spinach leaves has the lowest solubility how do you know?

The least soluble pigment is the yellow green chlorophyll B. You may be wondering why plants have more than one pigment. One reason is that these differently colored pigments absorb different colors of light: by having many pigments, plants can use more of the light energy shining down them.

What are the pigments in spinach leaves?

Spinach leaves contain chlorophyll a and b and β-carotene as major pigments as well. as smaller amounts of other pigments such as xanthophylls; these are oxidized versions of. carotenes and phenophytins, which look like chlorophyll except that the magnesium ion. (Mg+2) has been replaced by two hydrogen ions (H+).

What is the most nonpolar pigment found in spinach chloroplasts?

Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture (mobile phase) than to the paper. This stronger, nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts.

How to extract chlorophyll from spinach?

Tear the leaves into confetti-sized pieces and place them into a mortar. Add about 1.0 mL of acetone and grind the leaves with a pestle until the acetone turns a bright, deep green. You may add more acetone as necessary. So this way we can extract chlorophyll from Spinach .

What are the two types of pigments in leaves?

The leaves of plants contain a number of colored pigments generally falling into two categories, chlorophylls and carotenoids. The green chlorophylls a and b, which are highly conjugated compounds capture the (nongreen) light energy used in photosynthesis.