Table of Contents
- 1 Which tube contains plasma?
- 2 What color tube is serum?
- 3 How is serum obtained?
- 4 What tests are done on plasma?
- 5 How is serum obtained from the blood sample?
- 6 How is plasma converted to serum?
- 7 What kind of tubes are used for serum preparation?
- 8 What are the specimen requirements/containers for Serum separation?
Which tube contains plasma?
Send plasma in a plastic transport tube labeled “Plasma, Sodium Fluoride.” Send whole blood in a gray-top tube. Blue-top tube (also light blue-top tube): Contains sodium citrate. Be sure to use only tubes with a 3.2% sodium citrate concentration. These are easily identified by the yellow diagonal stripes on the label.
What color tube is serum?
PHLEBOTOMY SERVICES
| STOPPER COLOR | CONTENTS | VOL. |
|---|---|---|
| Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. . | ||
| Royal Blue | No additive (serum); special glass and stopper material | 7.0 mL |
| Red | No additive | 7.0 mL |
| Light Blue | 3.2% Sodium Citrate | 4.5 mL |
What is the difference between whole blood serum and plasma?
Serum is the liquid that remains after the blood has clotted. Plasma is the liquid that remains when clotting is prevented with the addition of an anticoagulant.
How do you obtain serum and plasma from a blood sample?
How to separate serum and plasma from blood. Serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot. The clot is removed by centrifugation and the resulting supernatant, designated serum, is carefully removed using a Pasteur pipette.
How is serum obtained?
To obtain serum, a blood sample is allowed to clot (coagulation). The sample is then centrifuged to remove the clot and blood cells, and the resulting liquid supernatant is serum.
What tests are done on plasma?
Plasma protein tests are blood tests that detect the amount of proteins in the blood. This lab work is usually ordered as part of a comprehensive metabolic panel (CMP) during a physical exam. The tests can help your doctor determine your overall health. Plasma protein tests are also known as a total protein test.
How can you tell the difference between serum and plasma?
A key difference between plasma and serum is that plasma is liquid, and serum is fluid. While most of the components are the same for both plasma and serum, plasma contains fibrinogen which is absent in serum. Serum is mostly used for blood typing but is also used for diagnostic testing.
How do you collect serum?
SERUM
- Perform venipuncture as with any other blood collection device.
- Invert the tube gently no more than eight times.
- Do not remove the stopper at any time.
- Centrifuge for at least 15 minutes at 2200-2500 RPM within one hour of collection.
- Transfer the serum to a plastic screw-cap vial for transport to the laboratory.
How is serum obtained from the blood sample?
How is plasma converted to serum?
Plasma can be converted to serum by the method of defibrination. Coagulation factors present in plasma can be activated to form fibrin, with the addition of calcium chloride and thrombin (1). Thrombin cleaves fibrinogen to form fibrin monomers, which polymerize, creating a stable clot.
What is the difference between plasma and serum tubes?
Serum Tubes. Don’t get serum confused with plasma – while they’re both the liquid, cell-free part of the blood which can be obtained by centrifugation, the key difference is that serum is the product of blood which has been allowed to clot, while in a plasma sample, the dense cells are simply spun to the bottom.
How do you separate serum and plasma from blood?
How to separate serum and plasma from blood. The clot is removed by centrifugation and the resulting supernatant, designated serum, is carefully removed using a Pasteur pipette. Plasma is produced when whole blood is collected in tubes that are treated with an anticoagulant. The blood does not clot in the plasma tube.
What kind of tubes are used for serum preparation?
Serum preparation. If commercially available tubes are to be used, the researcher should use the red topped tubes. These are available from Becton Dickinson (BD). BD’s trade name for the blood handling tubes is Vacutainer. After collection of the whole blood, allow the blood to clot by leaving it undisturbed at room temperature.
What are the specimen requirements/containers for Serum separation?
Specimen Requirements/Containers 1 Gold-top serum separator tube (SST) 2 Red-top tube, plastic 3 Red-top tube, glass 4 Pink-top tube (EDTA) 5 Light green-top tube (lithium heparin) 6 Dark green-top tube (sodium heparin) 7 Grey-top tube (potassium oxalate/sodium fluoride) 8 Lavender-top tube (EDTA) 9 Royal blue-top tube 10 Yellow-top tube (ACD)