Menu Close

Why the lenses of the microscope should always be clean after oil immersion observation of slide?

Why the lenses of the microscope should always be clean after oil immersion observation of slide?

IRREGULARITIES ON THE SLIDE AND ON THE STAGE OF THE MICROSCOPE ARE LARGE ENOUGH TO CAUSE CHANGES IN FOCUS. ONCE YOU ARE DONE VIEWING A SLIDE UNDER OIL IMMERSION, IT WILL BE NECESSARY TO CLEAN BOTH THE SLIDE AND THE OBJECTIVE.

Why do you use oil on a slide to be examined with the oil immersion objective?

It is best to use an oil-immersed objective at high magnification as the oil compensates for short focal lengths associated with larger magnifications. Oil is an ideal conduit in the preparation of slides because the refractive index is the same or similar as glass.

Why is it important to use a small amount of bacteria when preparing a smear?

General Considerations. Just as in preparing a smear, you only need a small amount of organism. If you have too many organisms, you won’t be able to see the morphology of individual cells.

What would most likely occur if you drenched the bacterial smear in alcohol for 1 minute quizlet?

What would most likely occur if you drenched the bacterial smear in alcohol for 1 minute during a Gram stain? All purple stain would escape as the cell wall of both Gram-positive and Gram-negative bacteria would dissolve.

Why must you always clean the microscope lenses with lens tissue?

Just like with the microscope frame, always clean and disinfect your optics immediately after use. Not only does this make the equipment safe and clean for other lab members, but it removes dust and contaminants that can affect observation, damage the lens surface, and impact image quality.

Why should the oil immersion objective be cleaned immediately after use?

Proper Use and Removal of Immersion Oil Even when employed properly, immersion oil must be removed immediately after use to prevent its accumulation in unwanted areas of the microscope, as well as to avoid optical degradation from dried oil residue on the objective.

Why oil is used in oil immersion lens?

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.

Why do we use oil in oil immersion lens?

The reason is that this oil replaces the air gaps between the condenser and the bottom of the slide and between the top of the slide or cover glass and the objective lens with a medium that has a refractive index equal to the lowest refractive index of these glass components.

Why is it important to air dry the bacteria before they are immobilized by heat fixation?

Why is it important to air dry the bacteria before they are immobilized by heat fixation? This ensures the optimal preservation of the bacterial morphology. Preparing smears disperses bacterial cells on a slide so that individual cells can be easily visualized under the microscope.

What is the importance of stain in microbiology?

The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.

How long will you wash the bacterial smear with alcohol?

5. Decolorize with 95% ethanol: let the alcohol run over surface of slide until no more crystal violet color comes out of the smear (time varies—no more than 5-10 seconds). 6. Rinse with water.

Why do you sterilize the wire loop?

It also ensures that any liquid culture on the loop will run down into the flame. c Sterilise a wire loop by heating to red hot in a roaring blue Bunsen burner flame before and after use. This ensures that contaminating bacterial spores are destroyed.

What happens when you remove oil from objective lens?

Dried oil is not only tough to remove from an objective lens, but this sticky substance can easily trap dust, dirt, fingerprints, and other contaminating particles. A little trapped dust can compromise image quality and cause optical errors like shade or fog.

Do you need to clean your objective lens?

Objectives are precision pieces of microscope equipment, so they must be cleaned to ensure proper optical performance. Despite this, researchers and lab technicians often forget to clean their immersion oil objective lenses—and end up facing some sticky consequences. Today, we will provide six tips to properly clean oil off your objective lens.

How can I get Dust Off my objective lens?

Use the eyepiece to inspect any dust on the objective. Objective lenses are usually very small, so it can be difficult to see any dust on the lens. Fortunately, we have a neat trick to overcome this challenge. Simply remove the eyepiece from the microscope and hold it up to the objective lens.

How can I Clean my microbus microscope lens?

It can be removed with lens paper dipped in a weak ammonia solution (one dropper full of household ammonia in 1/2 cup water). If you are using a 100x objective with immersion oil, just simply “swipe” the excess oil off the lens with a kimwipe after use.